Testosterone ELISA Kit 582701 from Cayman Chemical

Product Specs
ItemTestosterone ELISA Kit
CompanyCayman Chemical
Price Pricing Info Supplier Page View Company Product Page
Catalog Number582701
Quantity96 Solid
ApplicationsELISA
Detection Range3.9-500 pg/ml
Sensitivity6 pg/ml
Research AreaAndrogens, Endocrinology & Metabolism, Hormones & Receptors, Reproductive Biology

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Fecal microbial composition associated with testosterone in the development of Meishan male pigsFrontiers in MicrobiologyNovember 20, 2023Xueyuan Jiang, Shaoshan Deng, Naisheng Lu, Wen Yao, Dong Xia, Weilong Tu, Hulong Lei, Peng Jia, Yeqing Ganmeasured by using commercial enzyme-linked immunoassay (EIA) kits of testosterone (Cayman, Item No. 582701, inter-assay CV < 10.7%, intra-assay CV < 14.0%). 2.3 Anaerobic culture experiment of fecal bacteria

(2) Withaferin-A attenuates diabetes mellitus induced male reproductive dysfunction mediated by ERα in brain and testes of Swiss albino miceScientific ReportsOctober 17, 2023Kalpana Baghel, Zaffar Azam, Rashmi Srivastava, Neelima Gupta, Naveen Kango(ELISA) The estimation of testosterone, estradiol and insulin in serum was done by ELISA kits using EIA kit 582701, EIA kit 58225 and EIA kit 589501 respectively, as per the manufacturer’s instructions (Cayman Chemical,

(3) Testosterone identifies hatchling sex for Mojave desert tortoises (Gopherus agassizii)Scientific ReportsSeptember 8, 2023M. A. Walden, Kevin J. Loope, Elizabeth A. Hunter, Stephen J. Divers, Jessica R. Comolli, Todd C. Esque, Kevin T. Shoemakerall testosterone assays using the commercially-available Cayman Chemical Testosterone ELISA Kit (No. 582701, Ann Arbor, MI, USA), with a reported range of 3.9–500 pg/mL. To extract the free, unbound portion of

(4) Mating in the cold. Prolonged sperm storage provides opportunities for forced copulation by male bats during winterFrontiers in PhysiologySeptember 6, 2023Takahiro Sato, Toshie Sugiyama, Tsuneo Sekijimaconcentration was determined by ELISA Capture/Sandwich using a commercial kit (Testosterone ELISA kit, Item No. 582701, Cayman Chemical, Michigan, United States of America) (Puga et al., 2017). The assay was performed on

(5) Aire drives steroid hormone biosynthesis by medullary thymic epithelial cellsScience ImmunologyAugust 25, 2023Matthew D. Taves, Kaitlynn M. Donahue, Jing Bian, Margaret C. Cam, Jonathan D. Ashwell(corticosterone, Arbor Assays, K014; progesterone, Cayman Chemical, 582601; testosterone, Cayman Chemical, 582701; estradiol, Cayman Chemical, 501890) according to the manufacturer’s protocol, with standards diluted

(6) Zebrafish gonad mutant models reveal neuroendocrine mechanisms of brain sexual dimorphism and male mating behaviors of different brain regionsBiol Sex DifferAugust 21, 2023Xiangyan Dai, Ajay Pradhan, Jiao Liu, Ruolan Liu, Gang Zhai, Linyan Zhou, Jiyan Dai, Feng Shao, Zhiyong Yuan, Zhijian Wang, et al.11-ketotestosterone (11-KT), 17β-estradiol (E2)) were measured using ELISA kits (Cayman Chemical Company, 582701/582281/582751, Michigan, USA) according to the manufacturer’s instructions. For T and 11-KT assay, the

(7) Ablative radiotherapy improves survival but does not cure autochthonous mouse models of prostate and colorectal cancerCommunications MedicineAugust 9, 2023Daniel R. Schmidt, Iva Monique T. Gramatikov, Allison Sheen, Christopher L. Williams, Martina Hurwitz, Laura E. Dodge, Edward Holupka, W. S. Kiger, Milton R. Cornwall-Brady, Wei Huang, et al.measured by ELISA in 96-well plate format according to manufacturer recommendations (Cayman, Item # 582701). Absorbance (410 nm) was measured at 90 min on a Tecan infinite 200Pro. For standards, absorbance was

(8) Wound inflammation post-orchiectomy affects the social dynamic of Nelore bullsBMC Veterinary ResearchJuly 15, 2023Caique Marques Marcelino, Pedro Henrique Esteves Trindade, Henry David Mogollón García, Antonio Guilherme Roncada Pupulim, Cyntia Ludovico Martins, Guilherme Rizzoto, Francisco Teixeira-Neto, Fernanda Macitelli, John Patrick Kastelic, João Carlos Pinheiro Ferreiraconcentrations Plasma testosterone concentrations were measured with a commercial ELISA kit (Testosterone ELISA Kit®; #582701 – Cayman Chemical, Ann Arbor, MI, USA), with a 3.9 pg/mL sensitivity. Pre-castration samples were diluted

(9) Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signalingNPJ biofilms and microbiomesJuly 8, 2023Li S, Zhai J, Chu W, Geng X, Wang D, Jiao L, Lu G, Chan WY, Sun K, Sun Y, et al.(#MBS2018978, Mybiosource), Rat FSH ELISA Kit (#EKU04249, Biomatik, Ontario, Canada), Testosterone ELISA Kit (#582701, Cayman Chemical, Michigan), Rat SHBG ELISA Kit (#MBS261678, Mybiosource), Cortisol Parameter Assay Kit

(10) Seasonal Pattern of Advertisement Calling and Physiology in Prolonged Breeding Anurans, Japanese Tree Frog (Dryophytes japonicus)Animals (Basel)May 11, 2023Jun-Kyu Park, Yuno DoFinally, it was resuspended using the ELISA buffer provided by the 96-well testosterone ELISA kit (582701, Cayman Chemical, Ann Arbor, MI, USA). Plasma testosterone concentrations were assayed according to

(11) Microplastic Ingestion Induces Size-Specific Effects in Japanese QuailEnvironmental Science & TechnologyNovember 15, 2022Laura MonclÃºs, Eliana McCann Smith, Tomasz Maciej Ciesielski, Martin Wagner, Veerle L.B. Jaspersduplicate using competitive enzyme immunoassay for T and E2 following manufacturer protocols (product no. 582701 for T and 501890 for E2, Cayman, USA), with an intra-assay CV ≤ 11% (Table S4). 2.6 Statistics Statistical

(12) Biotin Enhances Testosterone Production in Mice and Their Testis-Derived CellsNutrientsNovember 10, 2022Kota Shiozawa, Misato Maeda, Hsin-Jung Ho, Tomoko Katsurai, Md. Zakir Hossain Howlader, Kimiko Horiuchi, Yumi Sugita, Yusuke Ohsaki, Afifah Zahra Agista, Tomoko Goto, et al.of the medium were determined using enzyme-linked immunosorbent assay (ELISA) kits (testosterone, #582701; progesterone, #582601; Cayman Chemical). Because both cells produce a relatively higher level of progesterone

(13) Magel2 knockdown in hypothalamic POMC neurons innervating the medial amygdala reduces susceptibility to diet-induced obesityLife science allianceNovember 1, 2022Choi Y, Min HY, Hwang J, Jo YH.levels were quantified using the ELISA kits (Cayman Chemical, 501890 for estradiol; Cayman Chemical, 582701 for testosterone; Thermo Fisher Scientific, KMC2281 for leptin, and Mercodia, 10-1247-01 for insulin,

(14) Curcumin Remedies Testicular Function and Spermatogenesis in Male Mice with Low-Carbohydrate-Diet-Induced Metabolic DysfunctionInternational journal of molecular sciencesSeptember 2, 2022Tsao CW, Ke PS, Yang HY, Chang TC, Liu CY.centrifuged at 14,000× g (4 °C) for 20 min. The testicular concentrations of testosterone (Cayman, Item No. 582701, Ann Arbor, MI, USA), 3β-HSD (MyBioSource, #3806055, San Diego, CA, USA) and 17β-HSD (MyBioSource, #3806056)

(15) Environmental conditions and male quality traits simultaneously explain variation of multiple colour signals in male lizardsJournal of Animal EcologySeptember 1, 2022Arnaud Badiane, AndrÃ©az DupouÃ©, Pauline Blaimont, Donald B. Miles, Anthony L. Gilbert, Mathieu Lerouxâ€Coyau, Anna Kawamoto, David Rozenâ€Rechels, Sandrine Meylan, Jean Clobert, et al.using colorimetric competitive enzyme immunoassays (Testosterone ELISA kit, Cayman Chemical Cat, ref. 582701). On the day of capture, we also counted the number of ticks present on the body of each individual,

(16) A comparison of non-surgical methods for sexing young gopher tortoises (Gopherus polyphemus)PeerJJune 14, 2022Kevin J. Loope, David C. Rostal, M.A. Walden, Kevin T. Shoemaker, Elizabeth A. Hunterenzyme-linked immunosorbent assay (ELISA). Samples were re-eluted the next morning in ELISA buffer (Item No. 582701, Cayman Chemical, Ann Arbor, MI) and vortexed for 20 seconds. We then performed ELISAs using the Cayman

(17) Characterization of the Interrenal Gland and Sexual Traits Development in cyp17a2-Deficient ZebrafishFront Endocrinol (Lausanne)June 6, 2022Shengchi Shi, Tingting Shu, Xi Li, Qiyong Lou, Xia Jin, Jiangyan He, Zhan Yin, Gang Zhai3 mpf were examined using commercial Enzyme Linked Immunosorbent Assay (ELISA) kits (P4: 582601, T: 582701, 11-KT: 582751, E2: 582251, and cortisol: 500360, Cayman Chemicals, Ann Arbor, MI, USA). The concentration

(18) Effects of long‐term exposure to TDCPP in zebrafish ( Danio rerio ) – Alternations of hormone balance and gene transcriptions along hypothalamus‐pituitary axesAnimal Models and Experimental MedicineJune 1, 2022Xiaoshan Liu, Xiaoxun Lu, Jiabin Hong, Jing Zhang, Juntong Lin, Mengzhu Jiang, Qian Liu, Kyungho Choi, Jingjing Zhangenzyme‐linked immunosorbent assay (ELISA) using commercially available kits (Cayman Chemical; T [Cat # 582701], E2 [Cat # 582251], 11‐KT [Cat # 582751). In zebrafish, plasma sex hormones were measured in male and

(19) Female presence does not increase testosterone but still ameliorates sickness behaviours in male Japanese quailRoyal Society Open ScienceMay 24, 2022Brenna M. G. Gormally, Kaelyn Bridgette, Aubrey Emmi, Delilah Schuerman, Patricia C. Lopeswere assayed for testosterone together using a previously validated [20] enzyme immunoassay kit (Item #582701, Cayman Chemical, Ann Arbor, MI, USA). Briefly, 5 µl of each plasma sample was diluted with 200 µl of

(20) Benign prostatic hyperplasia/obstruction ameliorated using a soluble guanylate cyclase activatorThe Journal of PathologyApril 1, 2022Irina V. Zabbarova, Youko Ikeda, Mark G. Kozlowski, Pradeep Tyagi, Lori A Birder, Basu Chakrabarty, Subashan KPG Perera, Rajiv Dhir, Adam C. Straub, Peter Sandner, et al.snap-frozen until used for assays. The cGMP (ab65356, Abcam, Waltham, MA, USA) and serum testosterone (582701, Cayman Chemicals, Ann Arbor, MI, USA) ELISAs were performed following the manufacturers’ recommendations. DNA

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

Testosterone ELISA Kit from Cayman Chemical

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Cayman Chemical

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Testosterone ELISA Kit from Cayman Chemical

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